精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Leishmania panamensis Lainson and Shaw
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Leishmania panamensis Lainson and Shaw
Leishmania panamensis Lainson and Shaw
規(guī)格:
貨期:
編號(hào):B240888
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Leishmania panamensis Lainson and Shaw
商品貨號(hào) B240888
Strain Designations MHOM/COL/81/L13
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Cutaneous lesion, Tumaco, Colombia, April 1985
Product Format frozen
Type Strain no
Comments
Evaluation of metastatic capability in hamsters
Medium ATCC® Medium 2736: M199, Modified Medium
Growth Conditions
Temperature: 20-25.0°C
Growth condition: axenic
Cryopreservation
1.?? Harvest cells from a culture that is at or near peak density by centrifugation at ~800 x g for 5 min. Pool the cell pellets into a single tube.

2.?? Adjust the concentration of cells to 2.0 x 107/ml.? If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.

3.?? Prepare a 10% (v/v) sterile DMSO solution in fresh medium as follows:? Add the required volume of DMSO to a glass screw-capped test tube and place on ice.? Allow the DMSO to solidify.? Add the required volume of refrigerated medium.? Dissolve the DMSO by inverting several times.? If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.

4.?? Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 107 and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.

5.?? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6. ?Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.

7.?? The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial enough to cover only the frozen material. Do not agitate the vial.

9.?? Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into 10.0 ml of fresh ATCC medium 2736.

10.????????? Incubate the tube at 20-25°C with the cap screwed on tightly.

Name of Depositor NG Saravia
Year of Origin 1985
References

Martinez JE, et al. Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters. J. Parasitol. 77: 762-768, 1991. PubMed: 1919926

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
盖州市| 麦盖提县| 曲沃县| 白玉县| 邻水| 黄龙县| 乌兰察布市| 罗甸县| 久治县| 沿河| 宣汉县| 闽清县| 姚安县| 石渠县| 洞口县| 通江县| 大安市| 南江县| 泰兴市| 肥城市| 伊金霍洛旗| 福泉市| 策勒县| 阿瓦提县| 宁国市| 株洲县| 灵川县| 任丘市| 天门市| 环江| 武强县| 江川县| 焦作市| 峡江县| 东丽区| 淄博市| 西盟| 鹤山市| 江山市| 多伦县| 武邑县|