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M1A
M1A
規(guī)格:
貨期:
編號(hào):B238558
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) M1A
商品貨號(hào) B238558
Designations M1A
Species Homo sapiens, human
Depositors J Mandel
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.099999904632568
Gene product: DNA Segment [DXS31]
Alleles: A1, A2,
Insert Size (kb) 2.100
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI/PstI--3.6, 2.1; HindIII--5.4, 0.23; PstI--5.6; HincII--5.6.
This probe was derived from a 7.5 genomic insert isolated from the chromosome X library of Davies et al. This probe contains a unique sequence (repeat free).
This probe detects a fragment mapping to Yq11 which is 80% homologous and is slightly smaller than 3.4 kb. Detection of the Y fragment can be prevented by washing at 65C in 0.5X SSC.
The fragment can also be isolated by double digestion with EcoRI and HhaI. The vector is in fragments less than 400 bp.
Enzyme(s) not detecting polymorphism: BamHI, BanI, BanII, BclI, BglI, BglII, BstNI, DraI, EcoRI, EcoRV, MspI, PvuII, SacI, ScaIl, StuI, TaqI, Tth111I, XbaI.
References

Wrogemann K, et al. An XmnI RFLP at the subtelomeric Xp locus DXS31. Nucleic Acids Res. 14: 4377, 1986. PubMed: 3714482

Oberle I, et al. Characterization of a set of X-linked sequences and of a panel of somatic cell hybrids useful for the regional mapping of the human X chromosome. Hum. Genet. 72: 43-49, 1986. PubMed: 3002952

Ross MT, et al. Long-range physical mapping around the human steriod sulfatase locus. Genomics 6: 528-539, 1990. PubMed: 2328992

Jean Louis Mandel, personal communication

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