精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pUB110cop1
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pUB110cop1
pUB110cop1
規(guī)格:
貨期:
編號(hào):B236565
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pUB110cop1
商品貨號(hào) B236565
Designations pUB110cop1
Depositors H Leonhardt
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pUB110cop1 (plasmid)
Promoters: Promoter none
Construction: pUB110, pEB114cop1
Marker(s):kanR,neoR
Construct size (kb): 4.5
Features: marker(s): kanR, neoR
promoter: none
replicon: pUB110
Applications
shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5.
The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability.
Low copy number shuttle vector.
Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130.
Media ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
清流县| 胶州市| 连山| 黄骅市| 额济纳旗| 郧西县| 颍上县| 循化| 信阳市| 株洲县| 车致| 马边| 汤阴县| 洪泽县| 井陉县| 六安市| 南汇区| 台湾省| 永春县| 滨海县| 苍溪县| 双峰县| 清苑县| 苍溪县| 湖南省| 惠州市| 梁山县| 田东县| 东乡族自治县| 岱山县| 荥经县| 东乌珠穆沁旗| 丽水市| 双江| 吴旗县| 青海省| 武乡县| 天全县| 拉萨市| 泾阳县| 密云县|