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Trichosphaerium sp.
Trichosphaerium sp.
規(guī)格:
貨期:
編號(hào):B236300
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Trichosphaerium sp.
商品貨號(hào) B236300
Strain Designations Am-I-7 wt (Amoeba-I-7 Wild Type)
Application
microorganisms for degrading plant cell walls
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
stipes of Sargassum muticum, Alegria Beach, Hollister Ranch, Santa Barbara Co., CA, 1984
Product Format test tube
Storage Conditions Test Tube: See handling procedure
Type Strain no
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
degradation of plant cell walls
digestion of seaweeds
Medium ATCC® Medium 1405: HESNW medium
Growth Conditions
Temperature: 25°C
Cryopreservation

Cryoprotective Solution
DMSO, 1.5 ml
Fresh growth, 8.5 ml

  1. Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.
  2. Harvest the culture by agitating the contents of each flask. A sterile cotton swab or cell scraper may be rubbed over the bottom surface of each flask to detach any adhering amoebae.  Transfer the cell suspensions to 15 ml plastic centrifuge tubes.
  3. Spin the cell suspensions at approximately 500 x g for 5 min.
  4. Pool the cell pellets and adjust the concentration to 2.0 - 4.0 x 107 cells/ml with a fresh ATCC medium 1405.
    *If the concentration is too low centrifuge at 500 x g for 5 min and resuspend in the volume of ATCC medium 1405 required to yield the desired concentration.
  5. Mix the cell preparation and 15% (v/v) DMSO in equal portions.  The final concentration will be 1.0 - 2.0 x 107 cells/ml and 7.5% DMSO.  The time from the mixing of the cell preparation and cryoprotective solution to the start of the freezing process should be no less than 15 min. and no more than 30 min.
  6. Dispense in 0.5 ml aliquots to 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  7. Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.  At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 2.5 to 3 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  
  8. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  9. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after thawed.
  10. Immediately after thawing, aseptically transfer contents to a T-25 tissue culture flask containing 10 ml ATCC medium 1405.
  11. Incubate in a 25°C incubator with the cap screwed on tightly.
  12. Once a healthy culture has been established, aseptically add 0.5 ml of heat-killed Dunaliella tertiolecta ATCC® 30929 (delayed addition of the food organism following the initial thaw seems to be beneficial).
  13. Follow the protocol for maintenance of culture.
Name of Depositor M Polne-Fuller
U.S. Patent Number
U.S. Patent
Year of Origin 1984
References

Polne-Fuller M. Microoganisms and methods for degrading plant cell walls and complex hydrocarbons. US Patent 5,413,933 dated May 9 1995

Polne-Fuller M. A multinucleated marine amoeba which digests seaweeds. J. Protozool. 34: 159-165, 1987.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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