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pMuTNF [0169]
pMuTNF [0169]
規(guī)格:
貨期:
編號(hào):B223907
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMuTNF [0169]
商品貨號(hào) B223907
Designations pMuTNF [0169]
GenBank Number

M11731

Species Mus musculus, mouse
Depositors B Fennie
Vector
Construct size (kb): 5.5
Insert
DNA: cDNA
Insert lengths(kb): 1.10099995136261
Tissue: monocyte-macrophage PU5-1.8 cell line (TIB 61)
Gene product: tumor necrosis factor, alpha (cachectin) [Tnfa]
Insert Size (kb) 1.101
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BglI--2.4, 1.6, 1.05, 0.45; EcoRI--4.5, 1.15; PvuII-- 2.8, 2.5, 0.3; SacI--5.6.
The insert contains the following restriction sites (nt from the 5' end): BglI--79, 389; SacI--709; PvuII--752.
The insert is the 5' EcoRI fragment from clone lambda6, and contains the 5' untranslated region, the complete coding sequence, and the 3' untranslated region up to the naturally occurring EcoRI site at nt 1105.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Pennica D, et al. Cloning and expression in Escherichia coli of the cDNA for murine tumor necrosis factor. Proc. Natl. Acad. Sci. USA 82: 6060-6064, 1985. PubMed: 3898078

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