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pNK2859
pNK2859
規(guī)格:
貨期:
編號:B222996
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pNK2859
商品貨號 B222996
Designations pNK2859
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors N Kleckner
Applications
Produces protein transposase, Tn10, ats1 ats2 (ATS)
Vector
Construct size (kb): 6.5
Insert
DNA: Genomic
Insert lengths (kb): 4.40
Gene product: transposase, Tn10, ats1 ats2 (ATS)
Target Gene: transposase, Tn10, ats1 ats2 (ATS)
Insert Size (kb) 4.40
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 2.1; HindIII--4.6, 1.2, 0.78; PstI--6.6.

This cassette is inserted into the HindIII site (original nt 2272) of the ATS transposase-containing EcoRI fragment of pNK2881, ATCC 77351.

Construct useful for general transposon mutagenesis in Escherichia coli.

pBR322 was modified by deletion of bp 75-2352 and destruction (by filling in) of the HindIII site.

Contains the ats1 ats2 transposase gene that permits relaxed insertion specificity (altered target specificity, ATS). Expression is regulated by the Ptac promoter, inducible by IPTG.

The transposase segment extends from IS10R to the EcoRI site at nt 3140 of Tn10, with a deletion of nucleotides 1329-1942 to remove the transposase binding site.

Contains a 1.8 kb mini-Tn10 cassette conferring kanamycin resistance, bounded by inverted repeats of the outermost 70 bp of IS10R and embedded in 40 bp of lambdacI terminating in HindIII sites.

Transcription from the Ptac promoter is in a direction opposite to that of amp.

The order of the major features in this plasmid is: EcoRI - Ptac - ATS transposase - mini-Tn10 kan - EcoRI - pMB1 ori - ampR.

Classification Enterobacteriaceae, Escherichia
References

Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561

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李經(jīng)理 13626845108 972239479
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