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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 6.8000001907348630

DESCRIPTION OF VECTOR:
Intact vector size: 6.800
Type of vector: phagemid
Cloning sites: SphI PstI SalI BamHI SmaI KpnI HpaI_
Polylinker sites: SphI PstI SalI BamHI SmaI KpnI HpaI
Other unique sites: ScaI SspI
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
replicon: ars1
replicon: pMB1
marker(s): LEU2
operator: tet, <-
promoter: CaMV, <-
marker(s): ampR, <-

Vector: pSLF101 (phagemid)

Promoters: Promoter CaMV

Marker(s):LEU2,ampR

Construct size (kb): 6.800000190734863

Features: marker(s): LEU2
marker(s): ampR
operator: tet
promoter: CaMV
replicon: ars1
replicon: pMB1

expression vector

Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.2; BamHI--6.8; EcoRI--3.9, 1.2.

The vector contains the constitutive CaMV promoter adjacent to the tet operator.

The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+.

A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437) has a tet repressor gene and integrating vector pSLF104 (ATCC 87621) contains the sup3-5 marker and tet repressor under adh promoter.

Temperature: 37.0°C

component of:ATCC 87685