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pEFD126.3 [RW-173]
pEFD126.3 [RW-173]
規(guī)格:
貨期:
編號:B221559
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pEFD126.3 [RW-173]
商品貨號 B221559
Designations pEFD126.3 [RW-173]
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs observed under normal stringency (0.1X SSC).
Vector
Construct size (kb): 7.0
Insert
DNA: genomic
Insert lengths(kb): 4.199999809265137
Gene product: DNA Segment, single copy [D9S7]
Insert Size (kb) 4.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; BamHI--4.3, 2.6; HindIII--7.0; BglI--2.9, 1.2, 1.0, 0.9, 0.6; AvaI--3.0, 2.3, 0.75, 0.7.
Codominant segregation has been demonstrated in 60 families.
Subcloned from cosmid EFD126 isolated using HBV-3 oligonucleotide. RFLPs observed under normal stringency (0.1X SSC).
References

Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pEFD126.3 on chromosome 9q (D9S7). Nucleic Acids Res. 15: 10607, 1987. PubMed: 2892177

Peter J O'Connell, personal communication

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