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VHE2.2 [VHE 2.2]
VHE2.2 [VHE 2.2]
規(guī)格:
貨期:
編號(hào):B216044
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) VHE2.2 [VHE 2.2]
商品貨號(hào) B216044
Designations VHE2.2 [VHE 2.2]
Species Homo sapiens, human
Depositors DW Cox
Vector
Construct size (kb): 6.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: immunoglobulin, heavy, variable, VH3 class [IGHV3*]
Alleles: D2, D1
Insert Size (kb) 2.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.25; PstI--4.75, 1.9; HincII--3.5 (doublet); BamHI--4.75, 2.0; HindII--6.8.
This probe identifies a VH3 gene subclass. This probe flanks p00803.
The approximate number of the VH3 class of genes is 24.
Detects polymorphisms with EcoRI, SacI, ApaI, HindIII, AvaII, RsaI, TaqI, and BglII. The greatest variation is seen with EcoRI.
There are three polymorphic loci detected with EcoRI: (1) 6.7 kb/no band; (2) 6.0 kb/4.6 kb; and (3) 3.5 kb/no band.
VHE2.2 is a subclone of lambda VH26.
References

Matthyssens G, Rabbitts TH. Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region. Proc. Natl. Acad. Sci. USA 77: 6561-6565, 1980. PubMed: 6450418

Walter MA, Cox DW. Analysis of genetic variation reveals human immunoglobulin VH-region gene organization. Am. J. Hum. Genet. 42: 446-451, 1988. PubMed: 2894757

Walter MA, et al. Apa I polymorphism of a human immunoglobin VH3 subclass locus. Nucleic Acids Res. 15: 4697, 1987. PubMed: 2884630

Diane W Cox, personal communication

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