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H16-L10-4R5
H16-L10-4R5
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貨期:
編號:B164523
品牌:Mingzhoubio

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產(chǎn)品名稱 H16-L10-4R5
商品貨號 B164523
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions liquid nitrogen vapor phase
Derivation
Mediastinal lymphocytes from BALB/c mice infected with influenzavirus A were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against influenzavirus A nucleoprotein
Cellular Products
immunoglobulin; monoclonal antibody; against influenzavirus A nucleoprotein
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 X 106 cells/mL.

Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Isotype IgG2a
Name of Depositor WU Gerhard
Deposited As mouse (B cell); mouse (myeloma)
References

Yewdell JW, et al. Expression of influenza A virus internal antigens on the surface of infected P815 cells. J. Immunol. 126: 1814-1819, 1981. PubMed: 7217668

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

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