精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > DII 33.1
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
DII 33.1
DII 33.1
規(guī)格:
貨期:
編號(hào):B164359
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DII 33.1
商品貨號(hào) B164359
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with carbohydrate moieties on the human insulin receptor.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human placental insulin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human placental insulin receptor
Tumorigenic Yes
Effects
Yes, in (BALB/c X SJL/J)F1 mice
Comments
Animals were immunized with Wheat Germ Agglutinin bound fraction of a placental membrane extract.
Spleen cells were fused with NS0 myeloma cells.
The antibody reacts with carbohydrate moieties on the human insulin receptor.
The antibody may cross-react with other cell surface glycoproteins.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing

    Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/ mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.

    Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
    Cryopreservation

    Complete growth medium described above supplemented with 10% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype IgG1
    Name of Depositor S Ganguly
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Ganguly S, et al. Insulin-dependent tyrosine protein kinase: cellular distribution and copurification with the insulin receptor. Curr. Top. Cell. Regul. 27: 83-94, 1985. PubMed: 3937668

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    梅經(jīng)理 17280875617 1438578920
    胡經(jīng)理 13345964880 2438244627
    周經(jīng)理 17757487661 1296385441
    于經(jīng)理 18067160830 2088210172
    沈經(jīng)理 19548299266 2662369050
    李經(jīng)理 13626845108 972239479
    大宁县| 昌都县| 枣庄市| 鄯善县| 南通市| 普兰县| 长汀县| 琼结县| 临海市| 浮山县| 昭平县| 安丘市| 平利县| 栾城县| 岫岩| 个旧市| 绵阳市| 宁强县| 图木舒克市| 洪湖市| 龙岩市| 壤塘县| 八宿县| 开江县| 雷波县| 安平县| 鹤壁市| 镇江市| 灵山县| 荃湾区| 白水县| 巴彦淖尔市| 林口县| 大英县| 洛南县| 宝山区| 武城县| 闽清县| 清丰县| 卓资县| 惠来县|