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BW5147.3(Thy-1-e).10
BW5147.3(Thy-1-e).10
規(guī)格:
貨期:
編號:B164060
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BW5147.3(Thy-1-e).10
商品貨號 B164060
Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain AKR/J
Applications
This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47).
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are deficient in dolichol-P-mannose synthetase.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Derivation
This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47).
Comments
This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are deficient in dolichol-P-mannose synthetase.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
Deposited As Mus musculus
References

Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934

Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. PubMed: 2880714

Chapman A, et al. The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose. J. Biol. Chem. 255: 4441-4446, 1980. PubMed: 7372584

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