精品久久久久久,在线观看精品国产,久久不卡,久久就是精品,国产精品无码久久久久,亚洲日本一区二区三区高清在线,亚洲午夜精品一级在线

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 90196B
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
90196B
90196B
規(guī)格:
貨期:
編號:B163848
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 90196B
商品貨號 B163848
Organism Homo sapiens, human
Tissue spleen
Product Format frozen
Morphology monocyte/macrophage
Culture Properties suspension
Biosafety Level 2  [Cells contain Epstein-Barr DNA viral sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The 90196B cell line was established from cryopreserved human spleen cells.
Comments
The cells tend to grow in floating clusters.
Single cells may become adherent.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing The cells tend to grow in floating clusters. Single cells may become adherent. Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 x 105 and 1x106cells/mL. Gently pipette to break large clusters into smaller clusters. Medium Renewal: Twice weekly
Cryopreservation
Culture medium,85%; DMSO,10%; extra FBS,10%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor E.I. du Pont de Nemours & Co.
Deposited As Homo sapiens
U.S. Patent Number
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
东港市| 长寿区| 南宁市| 承德市| 博客| 芷江| 洪江市| 巩留县| 阳泉市| 长子县| 萨迦县| 雷波县| 比如县| 大关县| 眉山市| 天全县| 西乌| 古蔺县| 隆回县| 云阳县| 黄平县| 永州市| 天津市| 恭城| 双牌县| 民丰县| 乌兰察布市| 沙湾县| 六安市| 荔浦县| 利川市| 棋牌| 永善县| 宝应县| 招远市| 徐闻县| 正定县| 平果县| 锡林浩特市| 岑溪市| 工布江达县|